Background:

Rabbit polyclonal antibody to Spot 14

Alternative names:

Thyroid hormone-inducible hepatic protein; Spot 14 protein; S14; SPOT14

Clonality:

Polyclonal

Species reactivity:

H

Host:

Rabbit

Immunogen:

Recombinant full length protein of human Spot 14

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IF/IC

Recommended dilution:

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of Spot 14 expression in mouse liver (A), rat brain (B), rat heart (C) whole cell lysates.

IHC description:

N/A

IF/ICC description:

Immunofluorescent analysis of Spot 14 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

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