Background:

Rabbit polyclonal antibody to ADPN

Alternative names:

ADPN; C22orf20; Patatin-like phospholipase domain-containing protein 3; Acylglycerol O-acyltransferase; Adiponutrin; Calcium-independent phospholipase A2-epsilon; iPLA2-epsilon

Clonality:

Polyclonal

Species reactivity:

H

Host:

Rabbit

Immunogen:

Recombinant full length protein of human ADPN

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IHC

Recommended dilution:

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of ADPN expression in Hela (A), mouse testis (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of ADPN staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

N/A

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

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