Background:

Mouse monoclonal antibody to HSP27

Alternative names:

HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27

Clonality:

Monoclonal

Species reactivity:

H

Host:

Mouse

Immunogen:

Recombinant protein corresponding to human HSP27.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Applications:

WB, IHC

Recommended dilution:

WB (1/1000 - 1/2000), IH (1/100 - 1/200)

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.

WB description:

Western blot analysis of HSP27 expression in Hela (A) whole cell lysates.

IHC description:

Immunohistochemical analysis of HSP27 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

N/A

IP description:

N/A

FC description:

N/A

ChIP description:

N/A

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com