Alternative names:

CLN1;PPT

Description:

Recombinant human PPT1 protein with C-terminal 6×His tag

Background:

The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2008]

Molecular characterization:

PPT1(Asp28-Gly306) 6×His tag

Molecular weight:

The protein has a predicted molecular mass 32.1 of kDa after removal of the signal peptide. The apparent molecular mass of PPT1-His is approximately 33-40 kDa due to glycosylation.

Tag:

C-6×His Tag

Expression host:

HEK293

Target:

PPT1

Purity:

The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.

Formulation / Reconstitution:

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

Storage:

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

More info:

Email info@sobekbio.com

Orders:

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