Alternative Name(s):

UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1 UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1

Species: (Organism)

Bos taurus (Bovine)

Gene Names:

B4GALT1

Tag info:

N-terminal GST-tagged

Target Protein AA Sequence:

MKFREPLLGGSAAMPGASLQRACR

Expression Region:

1-24aa

Subcellular Location:

Isoform Long: Golgi apparatus, Golgi stack membrane, Single-pass type II membrane protein, Cell membrane, Single-pass type II membrane protein, Cell surface, Cell projection, filopodium, Note=Found in trans cisternae of Golgi, B4GALT1 cell surface expression is regulated by UBE2Q1 (By similarity), SUBCELLULAR LOCATION: Isoform Short: Golgi apparatus, Golgi stack membrane, Single-pass type II membrane protein, Note=Found in trans cisternae of Golgi, SUBCELLULAR LOCATION: Processed beta-14-galactosyltransferase 1: Secreted

Tissue Specificity:

Protein Length:

Cytoplasmic Domain

Pathway:

Mol. Weight:

29.5 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Developmental Biology

Function:

The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.; FUNCTION

Involvement in disease:

Relevance:

The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Glycosyltransferase 7 family

Reference:

"Bovine galactosyltransferase: identification of a clone by direct immunological screening of a cDNA expression library." Shaper N.L., Shaper J.H., Meuth J.L., Fox J.L., Chang H., Kirsch I.R., Hollis G.F. Proc. Natl. Acad. Sci. U.S.A. 83:1573-1577(1986)