Alternative Name(s):

L-ascorbate peroxidase 1b ;APX1b ;AtAPx02

Species: (Organism)

Arabidopsis thaliana (Mouse-ear cress)

Gene Names:

APX2

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

KSYPEVKEEYKKAVQRCKRKLRGLIAEKHCAPIVLRLAWHSAGTFDVKTKTGGPFGTIRHPQELAHDANNGLDIAVRLLDPIKELFPILSYADFYQLAGVVAVEITGGPEIPFHPGRLDKVEPPPEGRLPQATKGVDHLRDVFGRMGLNDKDIVALSGGHTLGRCHKERSGFEGAWTPNPLIFDNSYFKEILSGEKEGLLQLPTDKALLDDPLFLPFVEKYAADEDAFFEDYTEAHLKLSELGFADK

Expression Region:

4-250aa

Subcellular Location:

Cytoplasm

Tissue Specificity:

Detected in bundle sheath cells, the photosynthetic cells that surround the phloem and xylem.

Protein Length:

Partial

Pathway:

Mol. Weight:

31.5 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Plays a key role in hydrogen peroxide removal.

Involvement in disease:

Relevance:

Plays a key role in hydrogen peroxide roval.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Peroxidase family, Ascorbate peroxidase subfamily

Reference:

Cytosolic ascorbate peroxidase from Arabidopsis thaliana L. is encoded by a small multigene family.Santos M., Gosseau H., Lister C., Foyer C., Creissen G.P., Mullineaux P.M.Planta 198:64-69(1996)