Alternative Name(s):

Short name:C1-THF synthase

Species: (Organism)

Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)

Gene Names:

MTHFD1

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

MAPAEILNGREISAQIRARLKNQVTQLKEQVPGFTPGLAILLVGNRDDSNLYINVKLKAAEEIGIKATHIKLPRTTTESEVIKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVDGLTSISAGKLARGDLNDCFIPCTPKGCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTANLDEEVNKGDILVVATGRPEMVKGEWIKPGAIVIDCGINYVPDDKKPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGKWMI

Expression Region:

1-305aa

Subcellular Location:

Cytoplasm

Tissue Specificity:

Protein Length:

Partial

Pathway:

Mol. Weight:

49 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Cell Biology

Function:

Involvement in disease:

Relevance:

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH. 5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate. ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Tetrahydrofolate dehydrogenase/cyclohydrolase family; Formate--tetrahydrofolate ligase family

Reference:

The German cDNA consortium Submitted (NOV-2004)