Alternative Name(s):

Telokin

Species: (Organism)

Gallus gallus (Chicken)

Gene Names:

Mylk

Tag info:

N-terminal 10xHis-SUMO-tagged and C-terminal MYC-tagged

Target Protein AA Sequence:

EVNYRTVTINTEQKVSDVYNIEERLGSGKFGQVFRLVEKKTGKVWAGKFFKAYSAKEKENIRDEISIMNCLHHPKLVQCVDAFEEKANIVMVLEMVSGGELFERIIDEDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTSIKLIDFGLARRLESAGSLKVLFGTPEFVAPEVINYEPIGYETDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKSRLNCTQCLQHPWLQKDTKNMEAKKLSK

Expression Region:

1435-1722aa

Subcellular Location:

Cytoplasm, cytosol, Membrane

Tissue Specificity:

Isoform telokin is expressed in gizzard, heart, lung, intestine, and skeletal muscle although the levels of the expression in the latter were much less than that in the gizzard.

Protein Length:

Partial

Pathway:

Mol. Weight:

52.9 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Signal Transduction

Function:

Phosphorylates a specific serine in the N-terminus of a myosin light chain, which leads to the formation of calmodulin/MLCK signal transduction complexes which allow selective transduction of calcium signals.

Involvement in disease:

Relevance:

Phosphorylates a specific serine in the N-terminus of a myosin light chain, which leads to the formation of calmodulin/MLCK signal transduction complexes which allow selective transduction of calcium signals.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Protein kinase superfamily, CAMK Ser/Thr protein kinase family

Reference:

"Multiple gene products are produced from a novel protein kinase transcription region." Watterson D.M., Collinge M., Lukas T.J., van Eldik L.J., Birukov K.G., Stepanova O.V., Shirinsky V.P. FEBS Lett. 373:217-220(1995)