Alternative Name(s):

Cell proliferation-inducing gene 46 protein;Cytokeratin-18 ;CK-18Keratin-18 ;K18

Species: (Organism)

Homo sapiens (Human)

Gene Names:

KRT18

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

ETENRRLESKIREHLEKKGPQVRDWSHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDAL

Expression Region:

102-396aa

Subcellular Location:

Cytoplasm, perinuclear region, Nucleus, nucleolus

Tissue Specificity:

Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

Protein Length:

Partial

Pathway:

Estrogensignalingpathway

Mol. Weight:

38 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Cell Cycle

Function:

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Involvement in disease:

Cirrhosis (CIRRH)

Relevance:

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells . When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma mbrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Intermediate filament family

Reference:

Comparison of mouse and human keratin 18 a component of intermediate filaments expressed prior to implantation.Oshima R.G., Millan J.L., Cecena G.Differentiation 33:61-68(1986)