Alternative Name(s):

(AQP-2)(ADH water channel)(Aquaporin-CD)(AQP-CD)(Collecting duct water channel protein)(WCH-CD)(Water channel protein for renal collecting duct)

Species: (Organism)

Rattus norvegicus (Rat)

Gene Names:

Aqp2

Tag info:

N-terminal 10xHis-tagged

Target Protein AA Sequence:

MWELRSIAFSRAVLAEFLATLLFVFFGLGSALQWASSPPSVLQIAVAFGLGIGILVQALGHVSGAHINPAVTVACLVGCHVSFLRAAFYVAAQLLGAVAGAAILHEITPVEIRGDLAVNALHNNATAGQAVTVELFLTMQLVLCIFASTDERRGDNLGSPALSIGFSVTLGHLLGIYFTGCSMNPARSLAPAVVTGKFDDHWVFWIGPLVGAIIGSLLYNYLLFPSAKSLQERLAVLKGLEPDTDWEEREVRRRQSVELHSPQSLPRGSKA

Expression Region:

1-271aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

30.4 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Signal Transduction

Function:

Involvement in disease:

Relevance:

Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient . Plays an essential role in renal water homeostasis .

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

"Expression, functional analysis, and in situ hybridization of a cloned rat kidney collecting duct water channel." Ma T., Hasegawa H., Skach W., Frigeri A., Verkman A.S. Am. J. Physiol. 266:C189-C197(1994)