Alternative Name(s):

(L1-ORF1p)(LINE retrotransposable element 1)(LINE1 retrotransposable element 1)(Transposase element L1Md-A101/L1Md-A102/L1Md-A2)

Species: (Organism)

Mus musculus (Mouse)

Gene Names:

Lire1

Tag info:

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Target Protein AA Sequence:

MAKGKRKNPTNRNQDHSPSSERSTPTPPSPGHPNTTENLDPDLKTFLMMMIEDIKKDFHKSLKDLQESTAKELQALKEKQENTAKQVMEMNKTILELKGEVDTIKKTQSEATLEIETLGKRSGTIDASISNRIQEMEERISGAEDSIENIDTTVKENTKCKRILTQNIQVIQDTMRRPNLRIIGIDENEDFQLKGPANIFNKIIEENFPNIKKEMPMIIQEAYRTPNRLDQKRNSSRHIIIRTTNALNKDRILKAVREKGQVTYKGRPIRITPDFSPETMKARRAWTDVIQTLREHKCQPRLLYPAKLSITIDGETKVFHDKTKFTQYLSTNPALQRIITEKKQYKDGNHALEQPRK

Expression Region:

1-357aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

48.7 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Involvement in disease:

Relevance:

Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. Functions as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

"Mobilization of LINE-1 retrotransposons is restricted by Tex19.1 in mouse embryonic stem cells." MacLennan M., Garcia-Canadas M., Reichmann J., Khazina E., Wagner G., Playfoot C.J., Salvador-Palomeque C., Mann A.R., Peressini P., Sanchez L., Dobie K., Read D., Hung C.C., Eskeland R., Meehan R.R., Weichenrieder O., Garcia-Perez J.L., Adams I.R. Elife 6:0-0(2017)