Alternative Name(s):

(Nucleocapsid protein)(Protein N)

Species: (Organism)

Porcine reproductive and respiratory syndrome virus (strain Lelystad) (PRRSV)

Gene Names:

N

Tag info:

C-terminal 6xHis-tagged

Target Protein AA Sequence:

MAGKNQSQKKKKSTAPMGNGQPVNQLCQLLGAMIKSQRQQPRGGQAKKKKPEKPHFPLAAEDDIRHHLTQTERSLCLQSIQTAFNQGAGTASLSSSGKVSFQVEFMLPVAHTVRLIRVTSTSASQGAS

Expression Region:

1-128aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

14.8 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Microbiology

Function:

Involvement in disease:

Relevance:

Multifunctional protein which associates with itself through both covalent and non-covalent interactions to provide the basis for viral assembly. May play a role in the regulation of cellular gene expression during infection by interacting with host HIC. Plays a role in the inhibition of the dsRNA-mediated IFN induction pathway by inhibiting host IRF3 phosphorylation and nuclear translocation.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

"Poly(A)-binding protein interacts with the nucleocapsid protein of porcine reproductive and respiratory syndrome virus and participates in viral replication." Wang X., Bai J., Zhang L., Wang X., Li Y., Jiang P. Antiviral Res. 96:315-323(2012)