Alternative Name(s):

(DRP-4)(Collapsin response mediator protein 3)(CRMP-3)(UNC33-like phosphoprotein 4)(ULIP-4)

Species: (Organism)

Homo sapiens (Human)

Gene Names:

DPYSL4

Tag info:

N-terminal 10xHis-GST-tagged and C-terminal V5-Myc-tagged

Target Protein AA Sequence:

TASLGTDGSHYWSKNWAKAAAFVTSPPVNPDPTTADHLTCLLSSGDLQVTGSAHCTFTTAQKAVGKDNFALIPEGTNGIEERMSMVWEKCVASGKMDENEFVAVTSTNAAKIFNFYPRKGRVAVGSDADLVIWNPKATKIISAKTHNLNVEYNIFEGVECRGAPAVVISQGRVALEDGKMFVTPGAGRFVPRKTFPDFVYKRIKARNRLAEIHGVPRGVYDGPVHEVMVPAKPGSGAPARASCPGKISVPPVRNLHQSGFSLSGSQADDHIARRTAQKIMA

Expression Region:

280-560aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Partial

Pathway:

Mol. Weight:

66.8 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Neuroscience

Function:

Involvement in disease:

Relevance:

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

"Amino- and carboxyl-terminal domains of Filamin-A interact with CRMP1 to mediate Sema3A signalling." Nakamura F., Kumeta K., Hida T., Isono T., Nakayama Y., Kuramata-Matsuoka E., Yamashita N., Uchida Y., Ogura K., Gengyo-Ando K., Mitani S., Ogino T., Goshima Y. Nat. Commun. 5:5325-5325(2014)