Alternative Name(s):

(Another partner for ARF 1)

Species: (Organism)

Mus musculus (Mouse)

Gene Names:

Znf410

Tag info:

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Target Protein AA Sequence:

MLSDELESKPELLVQFVQNTSIPLGQGLVESEAKDITCLSLLPVTEASECSRLMLPDETPNHANSSKEVPSSAVLRSLQVNVGPDGEETRAQTVQKSPEFLTTPESPSLLQDLQPSDSTSFILLNLTRAGLGSSAEHFVFVQDETEDSGADFLSAESTDSSIPWFLRVQELAHDSLIAATRAQLAKNAKTGSNGENVHLGSGDGQPKDSGPLPQAEKKLKCTVEGCDRTFVWPAHFKYHLKTHRNERSFICPAEGCGKSFYVLQRLKVHMRTHNGEKPFMCHESGCGKQFTTAGNLKNHRRIHTGEKPFLCEAQGCGRSFAEYSSLRKHLVVHSGEKPHQCQVCGKTFSQSGSRNVHMRKHHLQLGTTGSQEQDQTAEPLMGSSLLEDASVPNKNLVSMNSQSSLGGESLNLSNTNSILGVDDEVLTERASRPLSSVPDVTHHLVTMQSGRQSYEVSVLTAVNPQELLNQGDLTERQT

Expression Region:

1-478aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

59.7 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Involvement in disease:

Relevance:

Transcription factor that binds to the sequence motif 5'-CATCCCATAATA-3', and is specifically required to silence expression of fetal hemoglobin in adult erythroid cells. Prevents expression of fetal hemoglobin genes HBG1 and HBG2 through CHD4: acts as a direct transcriptional activator of CHD4, a central component of the NuRD complex that represses transcription of fetal hemoglobin genes HBG1 and HBG2 in erythroid cells. May also activate transcription of matrix-remodeling genes such as MMP1 during fibroblast senescence. May activate transcription of the gap junction gene GJC1, perhaps in response to increasing glucose. However, recent studies suggest that ZNF410 is dedicated to regulate expression of a single gene: CHD4.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

"ZNF410 represses fetal globin by singular control of CHD4." Vinjamur D.S., Yao Q., Cole M.A., McGuckin C., Ren C., Zeng J., Hossain M., Luk K., Wolfe S.A., Pinello L., Bauer D.E. Nat. Genet. 53:719-728(2021)