Alternative Name(s):

Species: (Organism)

Eucheuma serra (Marine red alga) (Sphaerococcus serra)

Gene Names:

N/A

Tag info:

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Target Protein AA Sequence:

GRYTVQNQWGGSSAPWNDAGLWILGSRGNQNVMAVDVNSSDGGANLNGTMTYSGEGPIGFKGARRGESNVYDVENQWGGSSAPWHAGGQFVIGSRSGQGVLAVNITSSDGGKTLTGTMTYEREGPIGFKGTQSGGDTYNVENQWGGSSAPWNKAGIWALGDRSGQAMIAMDVSSSDGGKTLEGTMQYKGEGPIGFRGKLSGANNYSVENQWGGSSAPWNAAGDWLIGDRHNQNITAVKVSSDNDGKNLDGTCTYEREGPIGFKGVATS

Expression Region:

1-268aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

35.4 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Involvement in disease:

Relevance:

Lectin specific for high mannose N-glycans, recognizes the branched moiety of these glycans. Does not recognize other types of N-glycans or monosaccharides. Agglutinates trypsin-treated sheep and rabbit erythrocytes and untreated sheep erythrocytes. Has mitogenic activity on mouse lymphocytes. Does not require metal ions for activity.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

"Strict specificity for high-mannose type N-glycans and primary structure of a red alga Eucheuma serra lectin." Hori K., Sato Y., Ito K., Fujiwara Y., Iwamoto Y., Makino H., Kawakubo A. Glycobiology 17:479-491(2007)