Alternative Name(s):

Cu-NIR

Species: (Organism)

Rhizobium sullae (Rhizobium hedysari)

Gene Names:

nirK

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

TPVRKLSATEIAALPRRKLDLVKPPFVHVHTQKAEGGPKVVEVTLTIEEKKLVIDGKGTEVNAMTFDGSVPGPLIVVHQDDYVEVTLVNPETNTLQHNIDFHSATGALGGGALTVVNPGESAVLRFKATKAGVFVYHCAPPGMVPWHVTSGMNGAIMVLPREGLTDGHGKELVYDKVYYLGEQDFYIPRDEKGEFKKYDSPGEAYEDTVAVMRTLTPTHIVFNGAVGALTGENALTAAVGERVLIVHSQANRDTRPHLIGGHGEYVWRTGKFVNVPDRDQETWFIPGPTRGAAYYTFEQPGIYAYVNHNLIEAFELGAAAHFKVTGDWNDDLMTTVRSPSGS

Expression Region:

36-377aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Full Length of Mature Protein

Pathway:

Mol. Weight:

40.8 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

others

Function:

Involvement in disease:

Relevance:

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

"Characterization of the gene encoding nitrite reductase and the physiological consequences of its expression in the nondenitrifying Rhizobium 'hedysari' strain HCNT1." Toffanin A., Wu Q., Maskus M., Caselia S., Abruna H.D., Shapleigh J.P. Appl. Environ. Microbiol. 62:4019-4025(1996)