Alternative Name(s):

Snake venom metalloproteinase von Willebrand factor-dependent platelet coagglutinin

Species: (Organism)

Bothrops jararaca (Jararaca) (Bothrops jajaraca)

Gene Names:

N/A

Tag info:

N-terminal 6xHis-KSI-tagged

Target Protein AA Sequence:

IISPPVCGNELLEEGEECDCGTPEN

Expression Region:

1-25aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

18.0 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

others

Function:

Involvement in disease:

Relevance:

Metalloproteinase that binds to von Willebrand factor (VWF) and induces its interaction with GPIb (GP1BA), resulting in platelet aggregation. Miscellaneous There are two distinct forms of the vWF-dependent platelet coagglutinin. The dimeric form (snaclec) is 34-fold more active than the metalloprotease botrocetin in promoting vWF binding to platelets (PubMed:1993206).

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

"Isolation and chemical characterization of two structurally and functionally distinct forms of botrocetin, the platelet coagglutinin isolated from the venom of Bothrops jararaca." Fujimura Y., Titani K., Usami Y., Suzuki M., Oyama R., Matsui T., Fukui H., Sugimoto M., Ruggeri Z.M. Biochemistry 30:1957-1964(1991)