Alternative Name(s):

SRY (Sex determining region Y)-box 1

Species: (Organism)

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Gene Names:

sox1

Tag info:

N-terminal 6xHis-tagged and C-terminal 6xHis-tagged

Target Protein AA Sequence:

MYSMMMETDLHSPGVQPPSNTGQTGGGGGNKANQDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKVMSEAEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLLKKDKYSLAGGLLHAAGGGHMGVGLSPGGGGGGTGGVVVQRLESPGSGASAGGYAHMNGWANGAYPGSVAAAAAAAMMQEAQLAYSQQQQQHPGSGGHHPHHHPHHPHHHPHHHPHHNPTPHPPPPPPQPMHRYDMSALQYSPLPGAQTYMTASPSGYGALGYSSSQQQHQGSPSSAAVAAAAAAAAAAAASSGALGALGSLVKSEPSVSPPVSGGGPHNRPPCPGDLREMISMYLPSGGEAGDPAAAAAAAAAAAAAATSRLHSLPQHYQGAGTGVTGTVPLTHI

Expression Region:

1-401aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

44.7 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Epigenetics and Nuclear Signaling

Function:

Involvement in disease:

Relevance:

Transcriptional activator. Participates in neural induction (By similarity).

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

"Sox3 acts through multiple regulatory targets to suppress Nodal signaling during Xenopus germ-layer specification." Zhang C., Grammer T.C., Basta T., Tai P., Espinosa J.M., Klymkowsky M.W. Submitted (DEC-2006)