Alternative Name(s):

AMBN; AMBN_HUMAN; Ameloblastin (enamel matrix protein); Ameloblastin

Species: (Organism)

Homo sapiens (Human)

Gene Names:

AMBN

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

VPFFPQQSGTPGMASLSLETMRQLGSLQRLNTLSQYSRYGFGKSFNSLWMHGLLPPHSSLPWMRPREHETQQYEYSLPVHPPPLPSQPSLKPQQPGLKPFLQSAAATTNQATALKEALQPPIHLGHLPLQEGELPLVQQQVAPSDKPPKPELPGVDFADPQGPSLPGMDFPDPQGPSLPGLDFADPQGSTIFQIARLISHGPMPQNKQSPLYPGMLYVPFGANQLNAPARLGIMSSEEVAGGREDPMAYGAMFPGFGGMRPGFEGMPHNPAMGGDFTLEFDSPVAATKGPENEEGGAQGSPMPEANPDNLENPAFLTELEPAPHAGLLALPKDDIPGLPRSPSGKMKGLPSVTPAAADPLMTPELADVYRTYDADMTTSVDFQEEATMDTTMAPNSLQTSMPGNKAQEPEMMHDAWHFQEP

Expression Region:

27-447aa

Subcellular Location:

Secreted, extracellular space, extracellular matrix

Tissue Specificity:

Ameloblast-specific. Located at the Tomes processes of secretory ameloblasts and in the sheath space between rod-interrod enamel.

Protein Length:

Full Length of Mature Protein

Pathway:

Mol. Weight:

47.3 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Signal Transduction

Function:

Involved in the mineralization and structural organization of enamel.

Involvement in disease:

Amelogenesis imperfecta 1F (AI1F)

Relevance:

Involved in the mineralization and structural organization of enamel.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Ameloblastin family

Reference:

"Cloning, characterization and immunolocalization of human ameloblastin."MacDougall M., Simmons D., Gu T.T., Forsman-Semb K., Mardh C.K., Mesbah M., Forest N., Krebsbach P.H., Yamada Y., Berdal A.Eur. J. Oral Sci. 108:303-310(2000)