Alternative Name(s):

Transcription activator VP30

Species: (Organism)

Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)

Gene Names:

VP30

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

MEASYERGRPRAARQHSRDGHDHHVRARSSSRENYRGEYRQSRSASQVRVPTVFHKKRVEPLTVPPAPKDICPTLKKGFLCDSSFCKKDHQLESLTDRELLLLIARKTCGSVEQQLNITAPKDSRLANPTADDFQQEEGPKITLLTLIKTAEHWARQDIRTIEDSKLRALLTLCAVMTRKFSKSQLSLLCETHLRREGLGQDQAEPVLEVYQRLHSDKGGSFEAALWQQWDRQSLIMFITAFLNIALQLPCESSAVVVSGLRTLVPQSDNEEASTNPGTCSWSDEGTP

Expression Region:

1-288aa

Subcellular Location:

Virion, Host cytoplasm

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

34.5 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA stem-loop at the transcription start site of the first gene. Binds to RNA (By similarity).

Involvement in disease:

Relevance:

Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA stem-loop at the transcription start site of the first gene. Binds to RNA (By similarity).

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Filoviridae minor nucleoprotein VP30 family

Reference:

"Crystal structure of the C-terminal domain of Ebola virus VP30 reveals a role in transcription and nucleocapsid association."Hartlieb B., Muziol T., Weissenhorn W., Becker S.Proc. Natl. Acad. Sci. U.S.A. 104:624-629(2007)