Alternative Name(s):

Antigen LB39-AAAntigen SK29-AAProtein Melan-A

Species: (Organism)

Homo sapiens (Human)

Gene Names:

MLANA

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

MPREDAHFIYGYPKKGHGHSYTTAEEAAGIGILTVILGVLLLIGCWYCRRRNGYRALMDKSLHVGTQCALTRRCPQEGFDHRDSKVSLQEKNCEPVVPNAPPAYEKLSAEQSPPPYSP

Expression Region:

1-118aa

Subcellular Location:

Endoplasmic reticulum membrane, Single-pass type III membrane protein, Golgi apparatus, Golgi apparatus, trans-Golgi network membrane, Melanosome

Tissue Specificity:

Expression is restricted to melanoma and melanocyte cell lines and retina.

Protein Length:

Full Length

Pathway:

Mol. Weight:

15.2 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Cancer

Function:

Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.

Involvement in disease:

Relevance:

Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

Cloning of the gene coding for a shared human melanoma antigen recognized by autologous T cells infiltrating into tumor.Kawakami Y., Eliyahu S., Delgado C.H., Robbins P.F., Rivoltini L., Topalian S.L., Miki T., Rosenberg S.A.Proc. Natl. Acad. Sci. U.S.A.91:3515-3519(1994)