Alternative Name(s):

ESAT-6

Species: (Organism)

Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

Gene Names:

esxA

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

WNFAGIEAAASAIQGNVTSIHSLLDEGKQSLTKLAAAWGGSGSEAYQGVQQKWDATATELNNALQNLARTISEAGQAMASTEGNVTGMFA

Expression Region:

6-95aa

Subcellular Location:

Secreted, Secreted, cell wall, Host cell surface, Host cytoplasm, Host endoplasmic reticulum, Host cell membrane

Tissue Specificity:

Protein Length:

Partial

Pathway:

Mol. Weight:

11.3 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

A secreted protein that plays a number of roles in modulating the host's immune response to infection as well as being responsible for bacterial escape into the host cytoplasm. Acts as a strong host (human) T-cell antigen

Involvement in disease:

Relevance:

Not known. Elicits high level of IFN-gamma from mory effector cells during the first phase of a protective immune response.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

WXG100 family, ESAT-6 subfamily

Reference:

Mutually dependent secretion of proteins required for mycobacterial virulence.Fortune S.M., Jaeger A., Sarracino D.A., Chase M.R., Sassetti C.M., Sherman D.R., Bloom B.R., Rubin E.J.Proc. Natl. Acad. Sci. U.S.A. 102:10676-10681(2005)