Alternative Name(s):

Alternative name(s): Allergen: Ber e 1 Cleaved into the following 2 chains: 2S sulfur-rich seed storage protein small chain 1 Alternative name(s): 2S albumin 1 small subunit 2S sulfur-rich seed storage protein large chain 1B Alternative name(s): 2S albumin 1 large subunit

Species: (Organism)

Brazil nut

Gene Names:

BE2S1

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

QEECREQMQRQQMLSHCRMYMRQQMEESPYQTMPRRGMEPHMSECCEQLEGMDESCRCEGLRMMMMRMQQEEMQPRGEQMRRMMRLAENIPSRCNLSPMRCPMGGS

Expression Region:

37-142aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Partial

Pathway:

Mol. Weight:

14.8 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Allergen

Function:

This is a 2S seed storage protein.

Involvement in disease:

Relevance:

This is a 2S seed storage protein.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

2S seed storage albumins family

Reference:

"The disulphide mapping, folding and characterisation of recombinant Ber e 1, an allergenic protein, and SFA8, two sulphur-rich 2S plant albumins."Alcocer M.J., Murtagh G.J., Bailey K., Dumoulin M., Meseguer A.S., Parker M.J., Archer D.B.J. Mol. Biol. 324:165-175(2002).