Alternative Name(s):

5-enolpyruvylshikimate-3-phosphate synthase 1 (EPSP synthase 1)

Species: (Organism)

Nicotiana tabacum (Common tobacco)

Gene Names:

EPSPS-1

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

KPNEIVLQPIKDISGTVKLPGSKSLSNRILLLAALSKGRTVVDNLLSSDDIHYMLGALKTLGLHVEDDNENQRAIVEGCGGQFPVGKKSEEEIQLFLGNAGTAMRPLTAAVTVAGGHSRYVLDGVPRMRERPIGDLVDGLKQLGAEVDCFLGTNCPPVRIVSKGGLPGGKVKLSGSISSQYLTALLMAAPLALGDVEIEIIDKLISVPYVEMTLKLMERFGVSVEHTSSWDKFLVRGGQKYKSPGKAYVEGDASSASYFLAGAAVTGGTVTVEGCGTSSLQGDVKFAEVLEKMGAEVTWTENSVTVKGPPRNSSGMKHLRAVDVNMNKMPDVAMTLAVVALFADGPTAIRDVASWRVKETERMIAICTELRKLGATVVEGSDYCIITPPEKLNVTEIDTYDDHRMAMAFSLAACADVPVTIKDPGCTRKTFPNYFDVLQQYSKH

Expression Region:

75-518aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Full Length of Mature Protein

Pathway:

Mol. Weight:

49.7 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Involvement in disease:

Relevance:

Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

"Expression and stability of amplified genes encoding 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate-tolerant tobacco cells." Wang Y., Jones J., Weller S., Goldsbrough P.B. Plant Mol. Biol. 17:1127-1138(1991)