Alternative Name(s):

NEC 1 Alternative name(s): Prohormone convertase 1 Proprotein convertase 1 Short name: PC1

Species: (Organism)

Rattus norvegicus (Rat)

Gene Names:

Pcsk1

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

SVPRDSALNLFNDPMWNQQWYLQDTRMTASLPKLDLHVIPVWQKGITGKGVVITVLDDGLEWNHTDIYANYDPEASYDFNDNDHDPFPRYDPTNENKHGTRCAGEIAMQANNHKCGVGVAYNSKVGGIRMLDGIVTDAIEASSIGFNPGHVDIYSASWGPNDDGKTVEGPGRLAQKAFEYGVKQGRQGKGSIFVWASGNGGRQGDNCDCDGYTDSIYTISISSASQQGLSPWYAEKCSSTLATSYSSGDYTDQRITSADLHNDCTETHTGTSASAPLAAGIFALALEANPNLTWRDMQHLVVWTSEYDPLANNPGWKKNGAGLMVNSRFGFGLLNAKALVDLADPRTWRNVPEKKECIIKDNNFEPRALKANGEVIVEIPTRACEGQENAINSLEHVQFEATIEYSRRGDLHVTLTSAAGTSTVLLAERERDTSPNGFKNWDFMSVHTWGENPVGTWTLKVTDMSGRMQNEGRIVNWKLILHGTSSQPEHMKQPRVYTSYNTVQNDRRGVEKMVNVVEEKPTQNSLNGNLLVPKNSSSSSVEDRRDEQVQGAPSKAMLRLLQSAFSKNTPSKQSSKIPSAKLSVPYEGLYEALEKLNKPSQLEDSEDSLYSDYVDVFYNTKPYKHRDDRLLQALMDILNEKN

Expression Region:

111-752aa

Subcellular Location:

Cytoplasmic vesicle, secretory vesicle

Tissue Specificity:

Protein Length:

Full Length of Mature Protein

Pathway:

Mol. Weight:

73.2 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Signal Transduction

Function:

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP.

Involvement in disease:

Relevance:

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Peptidase S8 family, Furin subfamily

Reference:

"Isolation of two complementary deoxyribonucleic acid clones from a rat insulinoma cell line based on similarities to Kex2 and furin sequences and the specific localization of each transcript to endocrine and neuroendocrine tissues in rats."Hakes D.J., Birch N.P., Mezey A., Dixon J.E.Endocrinology 129:3053-3063(1991).