Alternative Name(s):

AtriopeptidaseCommon acute lymphocytic leukemia antigen ;CALLAEnkephalinaseNeutral endopeptidase 24.11 ;NEP ;Neutral endopeptidase;Skin fibroblast elastase ;SFE; CD10

Species: (Organism)

Homo sapiens (Human)

Gene Names:

MME

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

YDDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDSRGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGLGQAYRAYQNYIKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCRVW

Expression Region:

52-750aa

Subcellular Location:

Cell membrane, Single-pass type II membrane protein

Tissue Specificity:

Protein Length:

Extracellular Domain

Pathway:

Renin-angiotensinsystem

Mol. Weight:

81.8 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Cancer

Function:

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids

Involvement in disease:

Charcot-Marie-Tooth disease 2T (CMT2T); Spinocerebellar ataxia 43 (SCA43)

Relevance:

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids . Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond . Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 . Involved in the degradation of atrial natriuretic factor (ANF) . Displays UV-inducible elastase activity toward skin preelastic and elastic fibers .

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Peptidase M13 family

Reference:

Common acute lymphocytic leukemia antigen is identical to neutral endopeptidase.Letarte M., Vera S., Tran R., Addis J.B.L., Onizuka R.J., Quackenbush E.J., Jongeneel C.V., McInnes R.R.J. Exp. Med. 168:1247-1253(1988)