Alternative Name(s):

Glpr

Species: (Organism)

Rattus norvegicus (Rat)

Gene Names:

Glp1r

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

GPRPQGATVSLSETVQKWREYRHQCQRFLTEAPLLATGLFCNRTFDDYACWPDGPPGSFVNVSCPWYLPWASSVLQGHVYRFCTAEGIWLHKDNSSLPWRDLSECEESKQGERNSPEEQLLSLY

Expression Region:

22-145aa

Subcellular Location:

Cell membrane, Multi-pass membrane protein

Tissue Specificity:

Pancreatic islets, stomach, lung, rat insulinoma cell line.

Protein Length:

Partial

Pathway:

Mol. Weight:

16.3 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Cardiovascular

Function:

G-protein coupled receptor for glucagon-like peptide 1 (GLP-1)

Involvement in disease:

Relevance:

G-protein coupled receptor for glucagon-like peptide 1 (GLP-1). Ligand binding triggers activation of a signaling cascade that leads to the activation of adenylyl cyclase and increased intracellular cAMP levels. Plays a role in regulating insulin secretion in response to GLP-1

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

G-protein coupled receptor 2 family

Reference:

"Mutations to the third cytoplasmic domain of the glucagon-like peptide 1 (GLP-1) receptor can functionally uncouple GLP-1-stimulated insulin secretion in HIT-T15 cells." Salapatek A.M., MacDonald P.E., Gaisano H.Y., Wheeler M.B. Unknown Journal 13:1305-1317(1999)