Alternative Name(s):

MIR-interacting saposin-like protein;Putative secreted protein Zsig9Transmembrane protein 4

Species: (Organism)

Homo sapiens (Human)

Gene Names:

CNPY2

Tag info:

N-terminal GST-tagged

Target Protein AA Sequence:

RSQDLHCGACRALVDELEWEIAQVDPKKTIQMGSFRINPDGSQSVVEVPYARSEAHLTELLEEICDRMKEYGEQIDPSTHRKNYVRVVGRNGESSELDLQGIRIDSDISGTLKFACESIVEEYEDELIEFFSREADNVKDKLCSKRTDLCDHALHISHDEL

Expression Region:

22-182aa

Subcellular Location:

Endoplasmic reticulum

Tissue Specificity:

Expressed in different tissues. Highest levels are detected in adult placenta, liver and pancreas.

Protein Length:

Full Length of Mature Protein

Pathway:

Mol. Weight:

45.4 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Metabolism

Function:

Positive regulator of neurite outgrowth by stabilizing myosin regulatory light chain (MRLC). It prevents MIR-mediated MRLC ubiquitination and its subsequent proteasomal degradation.

Involvement in disease:

Relevance:

Positive regulator of neurite outgrowth by stabilizing myosin regulatory light chain (MRLC). It prevents MIR-mediated MRLC ubiquitination and its subsequent proteasomal degradation.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Canopy family

Reference:

MSAP is a novel MIR-interacting protein that enhances neurite outgrowth and increases myosin regulatory light chain.Bornhauser B.C., Olsson P.-A., Lindholm D.J. Biol. Chem. 278:35412-35420(2003)