Alternative Name(s):

80K-H protein;Glucosidase II subunit betaProtein kinase C substrate 60.1KDA protein heavy chain ;PKCSH

Species: (Organism)

Homo sapiens (Human)

Gene Names:

PRKCSH

Tag info:

N-terminal GST-tagged

Target Protein AA Sequence:

VEVKRPRGVSLTNHHFYDESKPFTCLDGSATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYIPSNRVNDGVCDCCDGTDEYNSGVICENTCKEKGRKERESLQQMAEVTREGFRLKKILIEDWKKAREEKQKKLIELQAGKKSLEDQVEMLRTVKEEAEKPEREAKEQHQKLWEEQLAAAKAQQEQELAADAFKELDDDMDGTVSVTELQTHPELDTDGDGALSEAEAQALLSGDTQTDATSFYDRVWAAIRDKYRSEALPTDLPAPSAPDLTEPKE

Expression Region:

15-302aa

Subcellular Location:

Endoplasmic reticulum

Tissue Specificity:

Protein Length:

Partial

Pathway:

Proteinprocessinginendoplasmicreticulum

Mol. Weight:

59.3 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Signal Transduction

Function:

Regulatory subunit of glucosidase II.

Involvement in disease:

Polycystic liver disease 1 (PCLD1)

Relevance:

Regulatory subunit of glucosidase II.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

Isolation of cDNAs encoding a substrate for protein kinase C nucleotide sequence and chromosomal mapping of the gene for a human 80K protein.Sakai K., Masamichi H., Minoshima S., Kudoh J., Fukuyama R., Shimizu N.Genomics 5:309-315(1989)