Alternative Name(s):

ATP-dependent helicase CHD4Mi-2 autoantigen 218KDA protein;Mi2-beta

Species: (Organism)

Homo sapiens (Human)

Gene Names:

CHD4

Tag info:

N-terminal 6xHis-tagged and C-terminal 6xHis-tagged

Target Protein AA Sequence:

MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVES

Expression Region:

1-239aa

Subcellular Location:

Nucleus, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Tissue Specificity:

Protein Length:

Partial

Pathway:

Mol. Weight:

31.8 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Epigenetics and Nuclear Signaling

Function:

Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.

Involvement in disease:

Sifrim-Hitz-Weiss syndrome (SIHIWES)

Relevance:

Component of the histone deacetylase NuRD complex which participates in the rodeling of chromatin by deacetylating histones.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

SNF2/RAD54 helicase family

Reference:

The major dermatomyositis specific Mi-2 autoantigen is a presumed helicase involved in transcriptional activation.Seelig H.P., Moosbrugger I., Ehrfeld H., Fink T., Renz M., Genth E.Arthritis Rheum. 38:1389-1399(1995)