Alternative Name(s):

DIM1 dimethyladenosine transferase 1 homologDIM1 dimethyladenosine transferase 1-likeProbable 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferaseProbable 18S rRNA dimethylaseProbable S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase

Species: (Organism)

Homo sapiens (Human)

Gene Names:

DIMT1

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

MPKVKSGAIGRRRGRQEQRRELKSAGGLMFNTGIGQHILKNPLIINSIIDKAALRPTDVVLEVGPGTGNMTVKLLEKAKKVVACELDPRLVAELHKRVQGTPVASKLQVLVGDVLKTDLPFFDTCVANLPYQISSPFVFKLLLHRPFFRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSAVQQLLEKNYRIHCSVHNIIIPEDFSIADKIQQILTSTGFSDKRARSMDIDDFIRLLHGFNAEGIHFS

Expression Region:

1-313aa

Subcellular Location:

Nucleus, nucleoplasm, Nucleus, nucleolus

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

51.2 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Epigenetics and Nuclear Signaling

Function:

Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle

Involvement in disease:

Relevance:

Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Class I-like SAM-binding methyltransferase superfamily, rRNA adenine N(6)-methyltransferase family

Reference:

Full-insert sequence of mapped XREF EST.Barrow I.K.-P., Boguski M.S., Touchman J.W., Spencer F.Functional proteomic analysis of human nucleolus.Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.Mol. Biol. Cell 13:4100-4109(2002)