Alternative Name(s):

Vesicle transport v-SNARE protein Vti1-like 1Vti1-rp1

Species: (Organism)

Homo sapiens (Human)

Gene Names:

VTI1B

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

SSEHFEKLHEIFRGLHEDLQGVPERLLGTAGTEEKKKLIRDFDEKQQEANETLAEMEEELRYAPLSFRNPMMSKLRNYRKDLAKLHREVRSTPLTATPGGRGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATETDQIGSEIIEELGEQRDQLERTKSRLVNTSENLSKSRKILRSMSRKVTTNKLL

Expression Region:

7-208aa

Subcellular Location:

Early endosome membrane, Single-pass type IV membrane protein, Late endosome membrane, Single-pass type IV membrane protein, Lysosome membrane, Cytoplasmic granule, Recycling endosome membrane, Single-pass type IV membrane protein

Tissue Specificity:

Expressed in all tissues examined.

Protein Length:

Partial

Pathway:

Mol. Weight:

39.4 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Transport

Function:

V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence.

Involvement in disease:

Relevance:

V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target mbrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

VTI1 family

Reference:

A human homolog can functionally replace the yeast vesicle-associated SNARE Vti1p in two vesicle transport pathways.Fischer von Mollard G., Stevens T.H.J. Biol. Chem. 273:2624-2630(1998)