Alternative Name(s):

C20orf172; Chromosome 20 open reading frame 172; dJ469A13.2; DSN1; DSN1; MIND kinetochore complex component; homolog; DSN1; MIND kinetochore complex component; homolog (S. cerevisiae); DSN1; MIS12 kinetochore complex component; DSN1_HUMAN; FLJ13346; hKNL-3; Kinetochore associated protein DSN1; Kinetochore associated protein DSN1 homolog; Kinetochore null 3 homolog; Kinetochore-associated protein DSN1 homolog; KNL3; MGC32987; MIS13

Species: (Organism)

Homo sapiens (Human)

Gene Names:

DSN1

Tag info:

N-terminal GST-tagged

Target Protein AA Sequence:

MTSVTRSEIIDEKGPVMSKTHDHQLESSLSPVEVFAKTSASLEMNQGVSEERIHLGSSPKKGGNCDLSHQERLQSKSLHLSPQEQSASYQDRRQSWRRASMKETNRRKSLHPIHQGITELSRSISVDLAESKRLGCLLLSSFQFSIQKLEPFLRDTKGFSLESFRAKASSLSEELKHFADGLETDGTLQKCFEDSNGKASDFSLEASVAEMKEYITKFSLERQTWDQLLLHYQQEAKEILSRGSTEAKITEVKVEPMTYLGSSQNEVLNTKPDYQKILQNQSKVFDCMELVMDELQGSVKQLQAFMDESTQCFQKVSVQLGKRSMQQLDPSPARKLLKLQLQNPPAIHGSGSGSCQ

Expression Region:

1-356aa

Subcellular Location:

Nucleus, Chromosome, centromere, kinetochore

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

67.1 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Cell Biology

Function:

Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.

Involvement in disease:

Relevance:

Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

"A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1." Obuse C., Iwasaki O., Kiyomitsu T., Goshima G., Toyoda Y., Yanagida M. Nat. Cell Biol. 6:1135-1141(2004)