Alternative Name(s):

nadA; NMB0394; Quinolinate synthase A; EC 2.5.1.72

Species: (Organism)

Neisseria meningitidis serogroup B (strain MC58)

Gene Names:

nadA

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETADRLKDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAGVRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAAVKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYICRETGADMLLWQGSCIVHNEFKGQELAALKAEHPEAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVATDLGILHEMQKQAPDKQFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEILLDRKLGEAAKLPLQRMLDFAAGLKKKDVFNGMGPA

Expression Region:

1-370aa

Subcellular Location:

Cytoplasm

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

44.2 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.

Involvement in disease:

Relevance:

Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Quinolinate synthase A family, Type 1 subfamily

Reference:

"Expression of the meningococcal adhesin NadA is controlled by a transcriptional regulator of the MarR family." Schielke S., Huebner C., Spatz C., Nagele V., Ackermann N., Frosch M., Kurzai O., Schubert-Unkmeir A. Mol. Microbiol. 72:1054-1067(2009)