Alternative Name(s):

Exoenzyme T

Species: (Organism)

Aeromonas salmonicida

Gene Names:

aexT

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

MQIQANTVGTQAVAHHSDATTGVGRMGQMEARQVATGQDAILLGSRSEPQKGQGLLSRLGAQLARPFVAIKEWISNLLGTDKRAAAPKAQTAVSPEDLQRLMKQAAFGSSLGGFAKADVLNNITGEQLGKDHASLATGNGPLRSLCTALQAVVIGSQQPQLRELATGLLARPIAGIPLQQWGSVGGKVTELLTSAPPELLKEAMSQLHTAMGEVADLQRAVKAEVAGEPARSATTAAAVAPLQSGESEVNVEPADKALAEGLQEQFGLEAEQYLGEQPHGTYSDAEVMALGLYTNGEYQHLNRSLRQEKQLDAGQALIDQGMSTAFEKSTPTEQLIKTFRGTHGGDAFNEVAEGQVGHDVAYLSTSRDPKVATNFGGSGSISTIFGRSGIDVSDISVEGDEQEILYNKETDMRVLLSAKDERGVTRRVLEEASLGEQSGHSKGLLDGLDLARGAGGADKPQEQDIRLKMRGLDLA

Expression Region:

1-475aa

Subcellular Location:

Secreted

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

66.1 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Directly involved in the toxicity for RTG-2 (rainbow trout gonad) fish cells.

Involvement in disease:

Relevance:

Directly involved in the toxicity for RTG-2 (rainbow trout gonad) fish cells.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

YopE family

Reference:

The ADP-ribosylating toxin, AexT, from Aeromonas salmonicida subsp. salmonicida is translocated via a type III secretion pathway.Burr S.E., Stuber K., Frey J.J. Bacteriol. 185:6583-6591(2003)