Alternative Name(s):

Antigen mAgK114

Species: (Organism)

Mus musculus (Mouse)

Gene Names:

Plet1

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

SDNGSCVVLDNIYTSDILEISTMANVSGGDVTYTVTVPVNDSVSAVILKAVKEDDSPVGTWSGTYEKCNDSSVYYNLTSQSQSVFQTNWTVPTSEDVTKVNLQVLIVVNRTASKSSVKMEQVQPSASTPIPESSETSQTINTTPTVNTAKTTAKDTANTTAVTTANTTANTTAVTTAKTTAKSLAIRTLGS

Expression Region:

28-218aa

Subcellular Location:

Apical cell membrane, Lipid-anchor, GPI-anchor

Tissue Specificity:

Present in hair follicle cells and sebaceous gland of skin, ciliated epithelial cells of trachea and bronchial tube, striated portion of submandibular gland, distal convoluted tubule cells of kidney, ciliated epithelial cells of oviduct, medulla of adrenal gland and anterior lobe of pituitary gland. Expressed in keratinocytes of the hair follicle at the trichilemmal zone corresponding to the terminally differentiated outermost suprabasal outer root sheath (ORS), including that of the sebaceous gland duct (SGD) and the directly adjacent upper distal end of the companion layer (CL). Expression is similar in all hair follicle growth stages. Also detected during both the early and late anagen phases above the bulge of stem cells. Expressed at the leading edge of the epidermal wound. Not expressed in the interfollicular epidermis (IFE), inner root sheath (IRS) and hair fiber. Highly expressed in placenta. Detected in mammary and prostate epithelia and in the pancreas (at protein level).

Protein Length:

Full Length of Mature Protein

Pathway:

Mol. Weight:

36.1 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Cell Biology

Function:

Modulates leading keratinocyte migration and cellular adhesion to matrix proteins during a wound-healing response and promotes wound repair. May play a role during trichilemmal differentiation of the hair follicle.

Involvement in disease:

Relevance:

Modulates leading keratinocyte migration and cellular adhesion to matrix proteins during a wound-healing response and promotes wound repair. May play a role during trichilemmal differentiation of the hair follicle.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

"Expression of the orphan protein Plet-1 during trichilemmal differentiation of anagen hair follicles."Raymond K., Richter A., Kreft M., Frijns E., Janssen H., Slijper M., Praetzel-Wunder S., Langbein L., Sonnenberg A.J. Invest. Dermatol. 130:1500-1513(2010)