Alternative Name(s):

BUsg_347; Porin-like protein BUsg_347

Species: (Organism)

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)

Gene Names:

BUsg_347

Tag info:

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Target Protein AA Sequence:

IEIFNKNGNKLELYGSINPNHNFSNEFLSTKISSKEDNTNAILGLSGKIKITDKLSSYAQIEYKNNFFMPEDLMNKQQPNTVRLGYAGLKYGNLGSIDYGRNYGVIHDAQSLTDHVPYINKKSIFAYNDNYMVGRNHSLLTYRNNNVFGLVDGISFALQYQDEIKNRDLNKGKSSSGWGASLKYESDSGLTAVGSCFTSERMISSNKKDLKNTSVDSYGLGFKYDANNVYVAAFYGSARNLMPYNMHISDSFINETQNIEAIAEYSFDSGFHPSLSYLDSKGQNSNSPKKELDLAKQINISTRYEFNKNVSTYMNYKINLLKENDFISQNQIPTDNTIGAGVVYQF

Expression Region:

24-369aa

Subcellular Location:

Cell outer membrane, Multi-pass membrane protein

Tissue Specificity:

Protein Length:

Full Length of Mature Protein

Pathway:

Mol. Weight:

59 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Forms pores that allow passive diffusion of small molecules across the membrane.

Involvement in disease:

Relevance:

Forms pores that allow passive diffusion of small molecules across the membrane.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Gram-negative porin family

Reference:

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002)