Alternative Name(s):

NaD1

Species: (Organism)

Nicotiana alata (Winged tobacco) (Persian tobacco)

Gene Names:

D1

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

RECKTESNTFPGICITKPPCRKACISEKFTDGHCSKILRRCLCTKPC

Expression Region:

26-72aa

Subcellular Location:

Secreted, Vacuole

Tissue Specificity:

Most abundant in the epidermal cell layers of the petals and sepals, within the connective cells of the anthers, and the cortical cells of the style. Not detected in the tapetum, pollen mother cells, the transmitting tissue, the vascular bundles of the anther and style or in leaves. Expressed also in ovaries, but barley detectable in roots.

Protein Length:

Full Length of Mature Protein

Pathway:

Mol. Weight:

21.3 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Plant defense peptide with antifungal activity against F.oxysporum and B.cinerea. Retards the growth of the Lepidopteran insect pests H.armigera and H.punctigera.

Involvement in disease:

Relevance:

Plant defense peptide with antifungal activity against F.oxysporum and B.cinerea. Retards the growth of the Lepidopteran insect pests H.armigera and H.punctigera.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

DEFL family

Reference:

"The three-dimensional solution structure of NaD1, a new floral defensin from Nicotiana alata and its application to a homology model of the crop defense protein alfAFP." Lay F.T., Schirra H.J., Scanlon M.J., Anderson M.A., Craik D.J. J. Mol. Biol. 325:175-188(2003)