Alternative Name(s):

Susd4; Sushi domain-containing protein 4

Species: (Organism)

Mus musculus (Mouse)

Gene Names:

Susd4

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

FGPAQLTGGFDDLNVCADPGVPENGFRTPSGGVFFESSVTRFHCQDGFRLKGSTKRLCMKHFNGTLGWVPSDKPVCIQEDCRIPQIEDAEIRNKTYRHGEKLVIDCHEGFKIRYPDLYNLVSLCRDDGTWDNLPICQGCLRPLASSNGYVNISEFQTSFPVGTVIAYRCFPGFKLEGSENLECLHNLIWSSSPPRCLALEVCPLPPMVSHGDFICHPRPCERYNHGTVVEFYCDPGYSLTSDYKYITCQYGEWFPSYQVYCIKSEQTWPSTHETLLTT

Expression Region:

42-319aa

Subcellular Location:

Membrane, Single-pass type I membrane protein

Tissue Specificity:

High expression in brain and eye, with weaker expression in spinal cord and testis. Detected in white matter of brain and in the outer segments of photoreceptors.

Protein Length:

Partial

Pathway:

Mol. Weight:

35.5 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Cell Biology

Function:

Acts as complement inhibitor by disrupting the formation of the classical C3 convertase. Isoform 3 inhibits the classical complement pathway, while membrane-bound isoform 1 inhibits deposition of C3b via both the classical and alternative complement pathways.

Involvement in disease:

Relevance:

Acts as complement inhibitor by disrupting the formation of the classical C3 convertase. Isoform 3 inhibits the classical complement pathway, while membrane-bound isoform 1 inhibits deposition of C3b via both the classical and alternative complement pathways.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

"Quantitative Trait Locus Mapping of Macrophage Cholesterol Metabolism and CRISPR/Cas9 Editing Implicate an ACAT1 Truncation as a Causal Modifier Variant." Hai Q., Ritchey B., Robinet P., Alzayed A.M., Brubaker G., Zhang J., Smith J.D. Arterioscler. Thromb. Vasc. Biol. 38:83-91(2018)