Alternative Name(s):

Homolog-13WD repeat-containing and planar cell polarity effector protein

Species: (Organism)

Mus musculus (Mouse)

Gene Names:

Wdpcp

Tag info:

N-terminal GST-tagged

Target Protein AA Sequence:

MSFCLTELHLWSLKSTLHIADRDIGVYQYYDKKDPSVSATEHGNLEEKQRLAESRDYPWTLKNRRPEKLRDSLKELEELMQSSPCVLSKWKSKYICQLLFGSGVLVSLSLSGPQLEKVVIDRSLVGKLISDTISDALLTDSFIILSFLAQNKLCFIQFTKKMDSLDGNKRLEKLSALDLKISYYDIPGPANRTIDRHLAVNSTQDLVVCWWPLVSDDVWPWTPVSSEKDRANMLLLGFTQGGLEVLSFVRTEWSPLDVHFGTKQPYQVFTVECSVSVDKEPMADSCIYESVRNKLHCVSVTRIPLRSKAISCCRNSTEDKLIVGCEDSSVILYEAHRGVTLLAQAELRPSLISCHPSGAILLVGSNQGELQIFDIALSPINIQLLAEDYSPKETLQFKKFFDVSSSLVQMQWMAPPVVFQKPKRGEICDLLFLRFNKGPLGVLLFKLGILTRGQLGLVDLILQYIHYSEVYEAISILRSMDWDTLGQQCLIGMGTIVNHLLRQRLTPEREAQLEASLGTFYAPTRPLLDTTILEYREPVSKYARRLFHHLLRYKRFEKAFLLAVDIGARDLFMDIHYLALDMGELALAEVARRRAHDIDVESVSSGVELLGPLDRRDMLNEGFASSALMPEGENKFPGLLPSIGSTHMQTLQQKIPNGPSSRWAIERRTEEEEEEEEEEEEELCTDSSGATTWNAEGELKEDQRKQDIGDVGSLRMVHFGLV

Expression Region:

1-722aa

Subcellular Location:

Cell membrane, Cytoplasm, cytoskeleton, cilium axoneme, Cytoplasm, cytoskeleton, cilium basal body

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

108.7 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Probable effector of the planar cell polarity signaling pathway which regulates the septin cytoskeleton in both ciliogenesis and collective cell movements. Together with FUZ and WDPCP proposed to function as core component of the CPLANE (ciliogenesis and planar polarity effectors) complex involved in the recruitment of peripheral IFT-A proteins to basal bodies

Involvement in disease:

Relevance:

Probable effector of the planar cell polarity signaling pathway which regulates the septin cytoskeleton in both ciliogenesis and collective cell movents.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

WD repeat fritz family

Reference:

Comparative transcription map of the wobbler critical region on mouse chromosome 11 and the homologous region on human chromosome 2p13-14.Fuchs S., Resch K., Thiel C., Ulbrich M., Platzer M., Jockusch H., Schmitt-John T.BMC Genet. 3:14-14(2002)