Alternative Name(s):

27KDA inositol polyphosphate phosphatase interacting protein B ;IPIP27B

Species: (Organism)

Homo sapiens (Human)

Gene Names:

FAM109B

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

MKLNERSVAHYALSDSPADHMGFLRTWGGPGTPPTPSGTGRRCWFVLKGNLLFSFESREGRAPLSLVVLEGCTVELAEAPVPEEFAFAICFDAPGVRPHLLAAEGPAAQEAWVKVLSRASFGYMRLVVRELESQLQDARQSLALQRRSSWKSVASRCKPQAPNHRAAGLENGHCLSKDSSPVGLVEEAGSRSAGWGLAEWELQGPASLLLGKGQSPVSPETSCFSTLHDWYGQEIVELRQCWQKRAQGSHSKCEEQDRP

Expression Region:

1-259aa

Subcellular Location:

Early endosome, Recycling endosome, Golgi apparatus, trans-Golgi network, Cytoplasmic vesicle, clathrin-coated vesicle

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

44.3 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Cancer

Function:

Plays a role in endocytic trafficking. Required for receptor recycling from endosomes, both to the trans-Golgi network and the plasma membrane.

Involvement in disease:

Relevance:

Plays a role in endocytic trafficking. Required for receptor recycling from endosomes, both to the trans-Golgi network and the plasma mbrane.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Sesquipedalian family

Reference:

The PH domain proteins IPIP27A and B link OCRL1 to receptor recycling in the endocytic pathway.Noakes C.J., Lee G., Lowe M.Mol. Biol. Cell 22:606-623(2011)