Alternative Name(s):

Polydeoxyribonucleotide synthase [NAD(+)]

Species: (Organism)

Desulfotalea psychrophila (strain LSv54 / DSM 12343)

Gene Names:

ligA

Tag info:

N-terminal 10xHis-tagged

Target Protein AA Sequence:

RPQTSRLGRVNPVAEVEPTRLSGALIQRVTAHHYAMVRDRGIGPGARIRMSRSGEVIPKIEEVVKPVAAAQLDIPAHCPSCGSDLAWDGDYLFCLNNMSCPAQITNSMEHFFKILGNVDGFGPSSVQRLYEGGLVDLPALYAMESADFEQVGFGPKQATNMVEQLNRSREELIEDWRFLAAFGVHRLGMGNCEKLLRYVRLEDVFNLSEEQIVAEIKGFQEKTAHSICAGLAGIKDLFFQLFALGFKLEPTPLLAEGGQGEKSPISGKTIVFTGAMLAASRGDMEKQAKALGATVGKSITGKTDMLVTGQRVGATKMAKAETLGIAILSEEAYRQLVSLD

Expression Region:

340-679aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Partial

Pathway:

Mol. Weight:

40.4 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Epigenetics and Nuclear Signaling

Function:

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Involvement in disease:

Relevance:

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

NAD-dependent DNA ligase family, LigA subfamily

Reference:

"The genome of Desulfotalea psychrophila, a sulfate-reducing bacterium from permanently cold Arctic sediments." Rabus R., Ruepp A., Frickey T., Rattei T., Fartmann B., Stark M., Bauer M., Zibat A., Lombardot T., Becker I., Amann J., Gellner K., Teeling H., Leuschner W.D., Gloeckner F.-O., Lupas A.N., Amann R., Klenk H.-P. Environ. Microbiol. 6:887-902(2004)