Alternative Name(s):

Actinin-associated LIM protein;Alpha-actinin-2-associated LIM protein

Species: (Organism)

Homo sapiens (Human)

Gene Names:

PDLIM3

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

MPQTVILPGPAPWGFRLSGGIDFNQPLVITRITPGSKAAAANLCPGDVILAIDGFGTESMTHADAQDRIKAAAHQLCLKIDRGETHLWSPQVSEDGKAHPFKINLESEPQEFKPIGTAHNRRAQPFVAAANIDDKRQVVSASYNSPIGLYSTSNIQDALHGQLRGLIPSSPQKTGTTLNTSIIFGPNLS

Expression Region:

1-189aa

Subcellular Location:

Cytoplasm, myofibril, sarcomere, Z line

Tissue Specificity:

Isoform 1 is highly expressed in differentiated skeletal muscle. Isoform 2 is heart-specific.

Protein Length:

Full Length of Isoform 3

Pathway:

Mol. Weight:

36.2 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Signal Transduction

Function:

May play a role in the organization of actin filament arrays within muscle cells.

Involvement in disease:

Relevance:

May play a role in the organization of actin filament arrays within muscle cells.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

Actinin-associated LIM protein identification of a domain interaction between PDZ and spectrin-like repeat motifs.Xia H., Winokur S.T., Kuo W.-L., Altherr M.R., Bredt D.S.J. Cell Biol. 139:507-515(1997)