Alternative Name(s):

BP75 Seed biotinylated protein of 65KDA

Species: (Organism)

Glycine max (Soybean) (Glycine hispida)

Gene Names:

SBP65

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

MASEQLARRENTTTEKEIHVEKHRVPKMATHFEHLAEQAKESDITAGKDTPQGSIEALQAGERVKDHAGKAMGDIGGRGKARETHELGAHFESLADKVTDHAAANVVGNKESQREARGGVRDVGKFEMRTEGGEKGNKDRPELKTRTREVIGRTEKERGRESGGQVVAEKGRETETARGRVGAENEGARTTAVITCTLEKGGGTQKPIREEERESESERSAWEQISNYSDQATQGVKEKYERAKQAASETLNTTT

Expression Region:

1-255aa

Subcellular Location:

Tissue Specificity:

Expressed in the leaf primodium and the vascular tissues of the hypocotyl-radicle axis of mature seeds. High protein levels in dry and mature soybean seeds, but not found in fresh immature seeds.

Protein Length:

Full Length

Pathway:

Mol. Weight:

44 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Microbiology

Function:

May serve as a biotin provider for several growth-limiting enzymes that are necessary during seed development and the subsequent germination stages, and thus may play some roles in determining seed germination capacity.

Involvement in disease:

Relevance:

May serve as a biotin provider for several growth-limiting enzymes that are necessary during seed development and the subsequent germination stages, and thus may play some roles in determining seed germination capacity.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Seed biotin-containing protein SBP65 family

Reference:

"Tissue- and stage-specific expression of a soybean (Glycine max L.) seed-maturation, biotinylated protein."Hsing Y.-C., Tsou C.-H., Hsu T.-F., Chen Z.-Y., Hsieh K.-L., Hsieh J.-S., Chow T.-Y.Plant Mol. Biol. 38:481-490(1998)