Alternative Name(s):

HSP70 Heat shock 70 kDa protein Heat shock protein 70

Species: (Organism)

Cyanophora paradoxa

Gene Names:

dnaK-A

Tag info:

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Target Protein AA Sequence:

GEVKDIVLLDVTPLSLGVETLGGVMSKIIVRNTTIPTQKSELFSTATDGQTNVEIHVLQGERGFAKDNKSLGTFRLDGIAPAPRGVPQIEVTFDIDANGILSVTAKDKGTGKEKSITITGASTLSQDEVERMVQEAEKNAAQDKENRQKIELRNQAE

Expression Region:

377-533aa

Subcellular Location:

Plastid, cyanelle

Tissue Specificity:

Protein Length:

Partial

Pathway:

Mol. Weight:

21.9 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Signal Transduction

Function:

Acts as a chaperone.

Involvement in disease:

Relevance:

Acts as a chaperone.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Heat shock protein 70 family

Reference:

"The complete sequence of the cyanelle genome of Cyanophora paradoxa: the genetic complexity of a primitive plastid." Loeffelhardt W., Stirewalt V.L., Michalowski C.B., Annarella M., Farley J.Y., Schluchter W.M., Chung S., Newmann-Spallart C., Steiner J.M., Jakowitsch J., Bohnert H.J., Bryant D.A. (In) Schenk H.E.A., Herrmann R., Jeon K.W., Mueller N.E., Schwemmler W. (eds.); Eukaryotism and symbiosis, pp.40-48, Springer-Verlag, Heidelberg (1997)