Alternative Name(s):

Regulatory protein SIR2 homolog

Species: (Organism)

Xanthomonas campestris pv. campestris (strain B100)

Gene Names:

cobB

Tag info:

N-terminal 6xHis-SUMO-tagged

Target Protein AA Sequence:

MTVAITQTGPALQEFIKRHQRLFVLSGAGCSTDSGIPDYRDLHGGWKRPQPVTFQAFMGELSTRQRYWARSLVGWPRFGLARPNATHHALAALEARGQLELLLTQNVDRLHQAAGSQAVIDLHGRLDVVRCMGCEQRMPRTEFQLLLERDNPGWADLEAAQAPDGDADLDSVAFDNFVVPACPACGCVLKPDVVFFGENVPRERVERAFAHLQAADAVLVVGSSLMVYSGFRFVQAAARAGLPIAALNFGRTRADDLLSLKVEQSCAQALAFLQQPPDPLHTATARYHSARSA

Expression Region:

1-293aa

Subcellular Location:

Cytoplasm

Tissue Specificity:

Protein Length:

Full Length

Pathway:

Mol. Weight:

48.1 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

NAD-dependent protein deacetylase which modulates the activities of several enzymes which are inactive in their acetylated form.

Involvement in disease:

Relevance:

NAD-dependent protein deacetylase which modulates the activities of several enzymes which are inactive in their acetylated form. NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Sirtuin family, Class II subfamily

Reference:

"The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis."Vorhoelter F.-J., Schneiker S., Goesmann A., Krause L., Bekel T., Kaiser O., Linke B., Patschkowski T., Rueckert C., Schmid J., Sidhu V.K., Sieber V., Tauch A., Watt S.A., Weisshaar B., Becker A., Niehaus K., Puehler A.J. Biotechnol. 134:33-45(2008)