Alternative Name(s):

Vitamin B12-binding protein

Species: (Organism)

Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)

Gene Names:

btuF

Tag info:

Tag-Free

Target Protein AA Sequence:

LAPQPPAQHADADRACSFPVTEPDASNTAITLDSEPERVVTLNPSAAQTMWELGDRDAVVGVSQFGTYLPTASQRTVVSGGQPSQTNVEAVVGLDPDLVLAPNTVRNTTVTRLRSAGITVFQFRAATSIDGVVEKTATIGRLTGNCAAAAATTAEMRDRVAAIADAVPDTDSARPRVYYHLGDGYTAGPNTFIGAAIEAAGGHNIAADVNTTSSYPQLSEEVIVSQDPDVVVTGVSADRLDATASALVAPSSVVRNTTAYATGNVVAVNTNHINQPAPRIVEPMARMANAFHNTTINTTLDAQPSATTTATSTAPPTDAADGTAPGFGVAAAVCALAGAALVARR

Expression Region:

25-369aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Full Length of Mature Protein

Pathway:

Mol. Weight:

35.3 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Required for corrinoid utilization. Probably part of the ABC transporter complex BtuCDF involved in cobalamin (vitamin B12) import. Probably binds cobalamin and delivers it to the surface of BtuC (By similarity).

Involvement in disease:

Relevance:

Required for corrinoid utilization. Probably part of the ABC transporter complex BtuCDF involved in cobalamin import. Probably binds cobalamin and delivers it to the surface of BtuC.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Reference:

"Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1." Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K., Ruepp A., Soppa J., Tittor J., Oesterhelt D. Genomics 91:335-346(2008)