Alternative Name(s):

132 kDa crystal protein Crystaline entomocidal protoxin Insecticidal delta-endotoxin CryIF(b) cryIF(b), cryINA67-1

Species: (Organism)

Bacillus thuringiensis subsp. Morrisoni

Gene Names:

cry1Fb

Tag info:

N-terminal 6xHis-tagged

Target Protein AA Sequence:

VKGHVDVEEQNNHRSVLVVPEWEAEVSQEVRVCPGRGYILRVTAYKEGYGEGCVTIHEVDNNTDELKFSSNCEKEQVYPGNTVACNDYNKNHGANACSSRNGGYDESYESNSSIPADYAPVYEEEAYTDGQRGNPCEFNRGHTPLPAGYVTAELEYFPETDTVWVEIGETEGTFIVDSVELLLMEE

Expression Region:

984-1169aa

Subcellular Location:

Tissue Specificity:

Protein Length:

Partial

Pathway:

Mol. Weight:

26.3 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Promotes colloidosmotic lysis by binding to the midgut epithelial cells of insects.

Involvement in disease:

Relevance:

Promotes colloidosmotic lysis by binding to the midgut epithelial cells of insects.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Delta endotoxin family

Reference:

"A novel cry1Fb gene from Bacillus thuringiensis subsp. morrisoni." Song F., Zhang J., Ding Z., Chen Z., Li G., Huang D. Submitted (APR-1998)