Alternative Name(s):

3D9 antigen Adhesin 3

Species: (Organism)

Candida albicans (Yeast)

Gene Names:

ALS3

Tag info:

N-terminal 10xHis-B2M-tagged and C-terminal Myc-tagged

Target Protein AA Sequence:

VSVETESSTVTTAQTNPSVPTTESEVVFTTKGNNENGPYESPSTNVKSSMDENSEFTTSTAASTSTDIENETIATTGSVEASSPIISSSADETTTVTTTAESTSVIEQPTNNNGGGKAPSATSSPSTTTTANNDSVITGTTSTNQSQSQSQYNSDTQQTTLSQQMTSSLVSLHMLTTFDGSGSVIQHSTWLCGLITLLSLFI

Expression Region:

918-1119aa

Subcellular Location:

Cell membrane, Lipid-anchor, GPI-anchor, Secreted, cell wall

Tissue Specificity:

Protein Length:

Partial

Pathway:

Mol. Weight:

37.9 kDa

Purity:

Greater than 90% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Cell surface adhesion protein which mediates both yeast-to-host tissue adherence and yeast aggregation. Plays an important role in the biofilm formation and pathogenesis of C.albicans infections. Necessary for C.albicans to bind to N-cadherin on endothelial cells and E-cadherin on oral epithelial cells and subsequent endocytosis by these cells. During disseminated infection, mediates initial trafficking to the brain and renal cortex and contributes to fungal persistence in the kidneys.

Involvement in disease:

Relevance:

Cell surface adhesion protein which mediates both yeast-to-host tissue adherence and yeast aggregation. Plays an important role in the pathogenesis of C.albicans infections. Necessary for C.albicans to bind to N-cadherin on endothelial cells and E-cadherin on oral epithelial cells and subsequent endocytosis by these cells

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

ALS family

Reference:

"The peptide-binding cavity is essential for Als3-mediated adhesion of Candida albicans to human cells." Lin J., Oh S.H., Jones R., Garnett J.A., Salgado P.S., Rusnakova S., Matthews S.J., Hoyer L.L., Cota E. J. Biol. Chem. 289:18401-18412(2014)