Alternative Name(s):

Fetidin (Hemolysin) (Lysenin-3) (efL3) (LRP-2)

Species: (Organism)

Eisenia fetida (Red wiggler worm)

Gene Names:

N/A

Tag info:

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Target Protein AA Sequence:

MSSRAGIAEGYEQIEVDVVAVWKEGYVYENRGSTSVEQKIKITKGMRNLNSETKTLTASHSIGSTISTGDLFEIATVDVSYSYSHEESQVSMTETEVYESKEIEHTITIPPTSKFTRWQLNADVGGADIEYMYLIDEVTPIGGTLSIPQVIKSRAKILVGREIYLGETEIRIKHADRKEYMTVVSRKSWPAATLGHSKLYKFVLYEDMYGFRIKTLNTMYSGYEYAYSSDQGGIYFDQGSDNPKQRWAINKSLPLRHGDVVTFMNKYFTRSGLCYYDGPATDVYCLDKREDKWILEVVKP

Expression Region:

1-300aa

Subcellular Location:

Secreted, Target cell membrane

Tissue Specificity:

Expressed by coelomocytes.

Protein Length:

Full Length

Pathway:

Mol. Weight:

41.6 kDa

Purity:

Greater than 85% as determined by SDS-PAGE.

Form:

Liquid or Lyophilized powder

Buffer:

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Research Areas:

Others

Function:

Pore-forming toxin that specifically binds sphingomyelin in the plasma membrane of various cells (By similarity). Has hemolytic activity. It also has antibacterial activities against B.megaterium.

Involvement in disease:

Relevance:

Pore-forming toxin that specifically binds sphingomyelin in the plasma membrane of various cells. Has hemolytic activity. It also has antibacterial activities against B.megaterium.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Protein Families:

Lysenin family

Reference:

"Relationship between hemolytic molecules in Eisenia fetida earthworms." Prochazkova P., Silerova M., Felsberg J., Joskova R., Beschin A., De Baetselier P., Bilej M. Dev. Comp. Immunol. 30:381-392(2006)